<html> <head> <title> Lesson 12 </title> </head> <h1 align=center> Garlic Tutorial </h1> <h2 align=center> Lesson 12 - Replace Selected Residues </h2> <hr size="3"> In this lesson you will learn how to replace selected residues. The structure used in this example will be 1HUC.pdb, but you can use any other protein structure. <br><br> Before starting with this lesson, read something about the command <a href="../commands/rep.html"> REPLACE </a> . <br><br> Step 1 - start garlic (if not started already): <br><br> <font color=RED> garlic <br><br> </font> Step 2 - load the structure: <br><br> <font color=RED> load 1HUC.pdb <br><br> </font> Step 3 - change color: <br><br> <font color=RED> color cold <br><br> </font> Step 4 - select the residue 26 in the chain A: <br><br> <font color=RED> sel a/26/*/* <br><br> </font> Step 5 - define the replacement for this residue: <br><br> <font color=RED> seq = ser <br><br> </font> Step 6 - replace CYS 26 by SER: <br><br> <font color=RED> replace <br><br> </font> Move the residue 26 closer to check the replacement (use numeric keys). <br><br> Step 7 - discard structure: <br><br> <font color=RED> dis all <br><br> </font> <hr size="3"> </html>
